LOCUS       sequence-85030-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKOet-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             25..75
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             124..774
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatgggc gtggccgact tgatcaagaa gttcgagtcc
       61 atctccaagg aggaggggga tccaccggtc gccaccatgg tgagcaaggg cgaggagaat
      121 aacatggccg tgattaaacc agagatgaag atgaggtact acatggacgg ctccgtcaat
      181 gggcatgagt tcacaattga aggtgaaggc acaggcagac cttacgaggg acatcaagag
      241 atgacactac gcgtcacaat ggccaagggc gggccaatgc ctttcgcgtt tgacttagtg
      301 tcacacgtgt tctgttacgg ccacagacct tttactaaat atccagaaga gataccagac
      361 tatttcaaac aagcatttcc tgaaggcctg tcatgggaaa ggtcgttgga gttcgaagat
      421 ggtgggtccg cttcagtcag tgcgcatata agccttagag gaaacacctt ctaccacaaa
      481 tccaaattta ctggggttaa ctttcctgcc gatggtccta tcatgcaaaa ccaaagtgtt
      541 gattgggagc catcaaccga gaaaattact gccagcgacg gagttctgaa gggtgatgtt
      601 acgatgtacc taaaacttga aggaggcggc aatcacaaat gccaattcaa gactacttac
      661 aaggcggcaa aaaagattct taaaatgcca ggaagccatt acatcagcca tcgcctcgtc
      721 aggaaaaccg aaggcaacat tactgagctg gtagaagatg cagtagctca ttccggcggc
      781 atggacgagc tgtacaagta agcggccgcg actctagatc ataatcagcc ataccacatt
      841 tgtagaggtt ttacttgctt taaaaaaacc tcccacacct cccccctgaa cctgaaacat
      901 aaaatgaatg caattgttgt tgt
//
