LOCUS       sequence-85031-e        1543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: tdEos-N1
FEATURES             Location/Qualifiers
     source          1..1543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..93
                     /label=MCS
                     /note="multiple cloning site"
     CDS             101..778
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             827..1504
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atgagtgcga ttaagccaga
      121 catgaagatc aacctccgta tggaaggcaa cgtaaacggg caccactttg tgatcgacgg
      181 agatggtaca ggcaagcctt ttgagggaaa acagagtatg gatcttgaag tcaaagaggg
      241 cggacctctg ccttttgcct ttgatatcct gaccactgca ttccattacg gcaacagggt
      301 attcgccgaa tatccagacc acatacaaga ctattttaag cagtcgtttc ctaaggggta
      361 ttcgtgggaa cgaagcttga ctttcgaaga cgggggcatt tgcattgcca gaaacgacat
      421 aacaatggaa ggggacactt tctataataa agttcgattt cacggtgtaa actttcccgc
      481 caatggtcca gttatgcaga agaagacgct gaaatgggag ccctccactg agaaaatgta
      541 tgtgcgtgat ggagtgctga ctggtgatat tcgcatggct ttgttgcttg aaggaaatgc
      601 ccattaccga tgtgacttca gaactactta caaagctaag gagaagggtg tcaagttacc
      661 aggctaccac tttgtggacc actgcattga gattttaagc catgacaaag attacaacaa
      721 ggttaagctg tatgagcatg ctgttgctca ttctggattg cctgacaatg ccagacgatc
      781 cggtagcggt cacggtactg gttctactgg ttctggttct tctgagagtg cgattaagcc
      841 agacatgaag atcaacctcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga
      901 cggagatggt acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga
      961 gggcggacct ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag
     1021 ggtattcgcc gaatatccag accacataca agactatttt aagcagtcgt ttcctaaggg
     1081 gtattcgtgg gaacgaagct tgactttcga agacgggggc atttgcattg ccagaaacga
     1141 cataacaatg gaaggggaca ctttctataa taaagttcga tttcacggtg taaactttcc
     1201 cgccaatggt ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat
     1261 gtatgtgcgt gatggagtgc tgactggtga tattcgcatg gctttgttgc ttgaaggaaa
     1321 tgcccattac cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt
     1381 accaggctac cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa
     1441 caaggttaag ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgccagacg
     1501 ataagcggcc gcgactctag atcataatca gccataccac att
//
