LOCUS       sequence-85032-e         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 584, Emission: 607.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias 
            DA, Tsien RY
  TITLE     A monomeric red fluorescent protein.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7877-82.
  PUBMED    12060735
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2002-06-11"; volume: "99(12)"; pages: 
            "7877-82"
COMMENT     Sequence Label: mRFP1-N1
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..93
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..778
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             101..778
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(231..249)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     499..518
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     693..716
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    901..982
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(938..957)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggcctcct ccgaggacgt
      121 catcaaggag ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg gccacgagtt
      181 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
      241 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccctc agttccagta
      301 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
      361 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
      421 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
      481 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac
      541 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagatga ggctgaagct
      601 gaaggacggc ggccactacg acgccgaggt caagaccacc tacatggcca agaagcccgt
      661 gcagctgccc ggcgcctaca agaccgacat caagctggac atcacctccc acaacgagga
      721 ctacaccatc gtggaacagt acgagcgcgc cgagggccgc cactccaccg gcgcctaaag
      781 cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta
      841 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
      901 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
      961 aataaagcat ttttttcact gcattctagt tgt
//
