LOCUS       sequence-85033-e         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 585, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Bulina ME, Chudakov DM, Britanova OV, Yanushevich YG, Staroverov DB,
            Chepurnykh TV, Merzlyak EM, Shkrob MA, Lukyanov S, Lukyanov KA
  TITLE     A genetically encoded photosensitizer.
  JOURNAL   Nat Biotechnol. 2006 Jan;24(1):95-9. doi: 10.1038/nbt1175. Epub 2005
            Dec 20.
  PUBMED    16369538
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt1175"; 
            journalName: "Nat Biotechnol"; date: "2006-01"; volume: "24"; issue:
            "1"; pages: "95-9"
COMMENT     Sequence Label: KillerRed-N1
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..93
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(101..103,104..109,110..820)
                     /codon_start=1
                     /product="red fluorescent protein KillerRed, a 
                     genetically-encoded photosensitizer (Bulina et al., 2006)"
                     /label=KillerRed
                     /note="mammalian codon-optimized"
                     /translation="MGSEGGPALFQSDMTFKIFIDGEVNGQKFTIVADGSSKFPHGDFN
                     VHAVCETGKLPMSWKPICHLIQYGEPFFARYPDGISHFAQECFPEGLSIDRTVRFENDG
                     TMTSHHTYELDDTCVVSRITVNCDGFQPDGPIMRDQLVDILPNETHMFPHGPNAVRQLA
                     FIGFTTADGGLMMGHFDSKMTFNGSRAIEIPGPHFVTIITKQMRDTSDKRDHVCQREVA
                     YAHSVPRITSAIGSDED"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atgggttcag agggcggccc
      121 cgccctgttc cagagcgaca tgaccttcaa aatcttcatc gacggcgagg tgaacggcca
      181 gaagttcacc atcgtggccg acggcagcag caagttcccc cacggcgact tcaacgtgca
      241 cgccgtgtgc gagaccggca agctgcccat gagctggaag cccatctgcc acctgatcca
      301 gtacggcgag cccttcttcg cccgctaccc cgacggcatc agccatttcg cccaggagtg
      361 cttccccgag ggcctgagca tcgaccgcac cgtgcgcttc gagaacgacg gcaccatgac
      421 cagccaccac acctacgagc tggacgacac ctgcgtggtg agccgcatca ccgtgaactg
      481 cgacggcttc cagcccgacg gccccatcat gcgcgaccag ctggtggaca tcctgcccaa
      541 cgagacccac atgttccccc acggccccaa cgccgtgcgc cagctggcct tcatcggctt
      601 caccaccgcc gacggcggcc tgatgatggg ccacttcgac agcaagatga ccttcaacgg
      661 cagccgcgcc atcgagatcc ccggcccaca cttcgtgacc atcatcacca agcagatgag
      721 ggacaccagc gacaagcgcg accacgtgtg ccagcgcgag gtggcctacg cccacagcgt
      781 gccccgcatc accagcgcca tcggtagcga cgaggattaa gcggccgcga ctctagatca
      841 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaacctcc cacacctccc
      901 cctgaacctg aaacataaat gaatgcaatt gttgttgtta acttgttatt gcagcttata
      961 atgg
//
