LOCUS       sequence-85034-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Nguyen AW, Daugherty PS
  TITLE     Evolutionary optimization of fluorescent proteins for intracellular 
            FRET.
  JOURNAL   Nat Biotechnol. 2005 Mar;23(3):355-60. doi: 10.1038/nbt1066
  PUBMED    15696158
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2005-03"; volume: "23"; issue: "3"; pages: 
            "355-60"
COMMENT     Sequence Label: YPet-N1
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..93
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(101..103,104..106,107..820)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(407..426)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca aaggcgaaga
      121 gctgttcacc ggcgtggtgc ccatcctggt ggagctggac ggcgacgtga acggccacaa
      181 gttcagcgtg agcggcgagg gcgagggcga cgccacctac ggcaagctga ccctgaagct
      241 gctgtgcacc accggcaagc tgcccgtgcc ctggcccacc ctggtgacca ccctgggcta
      301 cggcgtgcag tgcttcgccc ggtaccccga ccacatgaag cagcacgact tcttcaagag
      361 cgccatgccc gagggctacg tgcaggagcg gaccatcttc ttcaaggacg acggcaacta
      421 caagacccgg gccgaggtga agttcgaggg cgacaccctg gtgaaccgga tcgagctgaa
      481 gggcatcgac ttcaaggagg acggcaacat cctgggccac aagctggagt acaactacaa
      541 cagccacaac gtgtacatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa
      601 gatccggcac aacatcgagg acggcggcgt gcagctggcc gaccactacc agcagaacac
      661 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagagcgc
      721 cctgttcaag gaccccaacg agaagcggga ccacatggtg ctgctggagt tcctgaccgc
      781 cgccggcatc accgagggca tgaacgagct ctataagtaa agcggccgcg actctagatc
      841 ataatcagcc ataccacatt tgtagaggtt t
//
