LOCUS       sequence-85035-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Nguyen AW, Daugherty PS
  TITLE     Evolutionary optimization of fluorescent proteins for intracellular 
            FRET.
  JOURNAL   Nat Biotechnol. 2005 Mar;23(3):355-60. doi: 10.1038/nbt1066
  PUBMED    15696158
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2005-03"; volume: "23"; issue: "3"; pages: 
            "355-60"
COMMENT     Sequence Label: CYPet-N1
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..93
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(101..103,104..106,107..820)
                     /codon_start=1
                     /product="cyan fluorescent protein optimized for FRET with 
                     YPet (Nguyen and Daugherty, 2005)"
                     /label=CyPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFGGIVPILVELEGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(407..426)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggagagga
      121 actgttcggc ggcatcgtgc ccatcctggt ggagctggag ggcgacgtga acggccacaa
      181 gttcagcgtg agcggcgagg gcgagggcga cgccacctac ggcaagctga ccctgaagtt
      241 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctggtgacca ccctgacctg
      301 gggcgtgcag tgcttcagcc ggtaccccga ccacatgaag cagcacgact tcttcaagag
      361 cgtgatgccc gagggctacg tgcaggagcg gaccatcttc ttcaaggacg acggcaacta
      421 caagacccgg gccgaggtga agttcgaggg cgacaccctg gtgaaccgga tcgagctgaa
      481 gggcatcgac ttcaaggagg acggcaacat cctgggccac aagctggagt acaactacat
      541 cagccacaac gtgtacatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa
      601 ggcccggcac aacatcaccg acggcagcgt gcagctggcc gaccactacc agcagaacac
      661 ccccatcggc gacggccccg tgatcctgcc cgacaaccac tacctgagca cccagagcgc
      721 cctgagcaag gaccccaacg agaagcggga ccacatggtg ctgctggagt tcgtgaccgc
      781 cgccggcatc acccacggca tggacgaact gtacaaataa agcggccgcg actctagatc
      841 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
      901 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
      961 tataatgg
//
