LOCUS       sequence-85039-e        1572 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien 
            RY
  TITLE     Improved monomeric red, orange and yellow fluorescent proteins 
            derived from Discosoma sp. red fluorescent protein.
  JOURNAL   Nat Biotechnol. 2004 Dec;22(12):1567-72. Epub 2004 Nov 21.
  PUBMED    15558047
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol."; date: "2004-12"; volume: "22(12)"; pages: "1567-72. 
            Epub 2004 Nov 21"
COMMENT     Sequence Label: tdTomato-N1
FEATURES             Location/Qualifiers
     source          1..1572
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    11..91
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             99..1529
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggactcag atctcgagct caagcttcga attctgcagt
       61 cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg
      121 tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt
      181 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
      241 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt
      301 acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct
      361 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg
      421 tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca
      481 ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca
      541 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc
      601 tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg
      661 tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg
      721 actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctggggc
      781 atggcaccgg cagcaccggc agcggcagct ccggcaccgc ctcctccgag gacaacaaca
      841 tggccgtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc
      901 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      961 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt
     1021 tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc
     1081 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg
     1141 tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc
     1201 gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     1261 cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc
     1321 tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga
     1381 agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca
     1441 acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc
     1501 tgtacggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat aatcagccat
     1561 accacattga ga
//
