LOCUS       sequence-85041-e        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 574, Emission: 596.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien 
            RY
  TITLE     Improved monomeric red, orange and yellow fluorescent proteins 
            derived from Discosoma sp. red fluorescent protein.
  JOURNAL   Nat Biotechnol. 2004 Dec;22(12):1567-72. Epub 2004 Nov 21.
  PUBMED    15558047
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol."; date: "2004-12"; volume: "22(12)"; pages: "1567-72. 
            Epub 2004 Nov 21"
COMMENT     Sequence Label: mStrawberry-N1
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..93
                     /label=MCS
                     /note="multiple cloning site"
     CDS             101..811
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /label=mStrawberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILTPNFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVELYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(246..264)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     514..533
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    933..1014
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(970..989)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcgggccc gggatccacc ggtggctacc atggtgagca agggcgagga
      121 gaataacatg gccatcatca aggagttcat gcgcttcaag gtgcgcatgg agggctccgt
      181 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
      241 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctaac
      301 ccccaacttc acctacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
      361 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
      421 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
      481 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
      541 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
      601 gatgaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
      661 ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc ggcatcaagt tggacatcac
      721 ctcccacaac gaggactaca ccatcgtgga actgtacgaa cgcgccgagg gccgccactc
      781 caccggcggc atggacgagc tgtacaagta agcggccgcg actctagatc ataatcagcc
      841 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
      901 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
      961 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     1021 gttgtg
//
