LOCUS       sequence-85043-e        1543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: tdEos-C1
FEATURES             Location/Qualifiers
     source          1..1543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..712
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             761..1435
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     misc_feature    1436..1501
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatgagt gcgattaagc cagacatgaa
       61 gatcaacctc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg acggagatgg
      121 tacaggcaag ccttttgagg gaaaacagag tatggatctt gaagtcaaag agggcggacc
      181 tctgcctttt gcctttgata tcctgaccac tgcattccat tacggcaaca gggtattcgc
      241 cgaatatcca gaccacatac aagactattt taagcagtcg tttcctaagg ggtattcgtg
      301 ggaacgaagc ttgactttcg aagacggggg catttgcatt gccagaaacg acataacaat
      361 ggaaggggac actttctata ataaagttcg atttcacggt gtaaactttc ccgccaatgg
      421 tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa tgtatgtgcg
      481 tgatggagtg ctgactggtg atattcgcat ggctttgttg cttgaaggaa atgcccatta
      541 ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt taccaggcta
      601 ccactttgtg gaccactgca ttgagatttt aagccatgac aaagattaca acaaggttaa
      661 gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac gatccggtag
      721 cggtcacggt actggttcta ctggttctgg ttcttctgag agtgcgatta agccagacat
      781 gaagatcaac ctccgtatgg aaggcaacgt aaacgggcac cactttgtga tcgacggaga
      841 tggtacaggc aagccttttg agggaaaaca gagtatggat cttgaagtca aagagggcgg
      901 acctctgcct tttgcctttg atatcctgac cactgcattc cattacggca acagggtatt
      961 cgccgaatat ccagaccaca tacaagacta ttttaagcag tcgtttccta aggggtattc
     1021 gtgggaacga agcttgactt tcgaagacgg gggcatttgc attgccagaa acgacataac
     1081 aatggaaggg gacactttct ataataaagt tcgatttcac ggtgtaaact ttcccgccaa
     1141 tggtccagtt atgcagaaga agacgctgaa atgggagccc tccactgaga aaatgtatgt
     1201 gcgtgatgga gtgctgactg gtgatattcg catggctttg ttgcttgaag gaaatgccca
     1261 ttaccgatgt gacttcagaa ctacttacaa agctaaggag aagggtgtca agttaccagg
     1321 ctaccacttt gtggaccact gcattgagat tttaagccat gacaaagatt acaacaaggt
     1381 taagctgtat gagcatgctg ttgctcattc tggattgcct gacaatgcca gacgatccgg
     1441 actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc
     1501 caccggatct agataactga tcataatcag ccataccaca ttt
//
