LOCUS       sequence-85044-e        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mEos-A69V-C1
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..712
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFVEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     misc_feature    713..778
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    902..983
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(939..958)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatgagt gcgattaagc cagacatgaa
       61 gatcaacctc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg acggagatgg
      121 tacaggcaag ccttttgagg gaaaacagag tatggatctt gaagtcaaag agggcggacc
      181 tctgcctttt gcctttgata tcctgaccac tgcattccat tacggcaaca gggtattcgt
      241 cgaatatcca gaccacatac aagactattt taagcagtcg tttcctaagg ggtattcgtg
      301 ggaacgaagc ttgactttcg aagacggggg catttgcatt gccagaaacg acataacaat
      361 ggaaggggac actttctata ataaagttcg atttcacggt acaaactttc ccgccaatgg
      421 tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa tgtatgtgcg
      481 tgatggagtg ctgacgggtg atattcacat ggctttgttg cttgaaggaa atgcccatta
      541 ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt taccaggcta
      601 ccactttgtg gaccactgca ttgagatttt aagccatgac aaagattaca acaaggttaa
      661 gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac gatccggact
      721 cagatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac
      781 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt
      841 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
      901 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
      961 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaactcat catgtatctt
     1021 aacgcgtaaa ttgtagcgtt atattttgtt aaattcgcg
//
