LOCUS       sequence-85046-e        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Nguyen AW, Daugherty PS
  TITLE     Evolutionary optimization of fluorescent proteins for intracellular 
            FRET.
  JOURNAL   Nat Biotechnol. 2005 Mar;23(3):355-60. doi: 10.1038/nbt1066
  PUBMED    15696158
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2005-03"; volume: "23"; issue: "3"; pages: 
            "355-60"
COMMENT     Sequence Label: CYPet-C1
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="cyan fluorescent protein optimized for FRET with 
                     YPet (Nguyen and Daugherty, 2005)"
                     /label=CyPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFGGIVPILVELEGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    752..817
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    940..1018
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggag aggaactgtt
       61 cggcggcatc gtgcccatcc tggtggagct ggagggcgac gtgaacggcc acaagttcag
      121 cgtgagcggc gagggcgagg gcgacgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctggtg accaccctga cctggggcgt
      241 gcagtgcttc agccggtacc ccgaccacat gaagcagcac gacttcttca agagcgtgat
      301 gcccgagggc tacgtgcagg agcggaccat cttcttcaag gacgacggca actacaagac
      361 ccgggccgag gtgaagttcg agggcgacac cctggtgaac cggatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg ccacaagctg gagtacaact acatcagcca
      481 caacgtgtac atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaaggcccg
      541 gcacaacatc accgacggca gcgtgcagct ggccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgatcc tgcccgacaa ccactacctg agcacccaga gcgccctgag
      661 caaggacccc aacgagaagc gggaccacat ggtgctgctg gagttcgtga ccgccgccgg
      721 catcacccac ggcatggacg aactgtacaa atccggactc agatctcgag ctcaagcttc
      781 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat
      841 aatcagccat accacatttg tagaggtttt acttgcttta aaaacctccc acacctcccc
      901 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
      961 aatggttaca aataagcaat agcatcacaa tttcacaata aagcattttt ttcactgcat
     1021 tcta
//
