LOCUS       sequence-85052-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Dronpa3-C1
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..709
                     /codon_start=1
                     /product="photoswitchable Dronpa-Green3 fluorescent 
                     protein"
                     /label=Dronpa-Green3
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDINAALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     misc_feature    710..775
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agtgtgatta aaccagacat
       61 gaagatcaag ctgcgtatgg aaggcgctgt aaatggacac ccgttcgcga ttgaaggagt
      121 tggccttggg aagcctttcg agggaaaaca gagtatggac cttaaagtca aagaaggcgg
      181 acctctgcct ttcgcctatg acatcttgac aactgtgttc tgttacggca acagggtatt
      241 cgccaaatac ccagaaaata tagtagacta tttcaagcag tcgtttcctg agggctactc
      301 ttgggaacga agcatgaatt acgaagacgg gggcatttgt aacgcgacaa acgacataac
      361 cctggatggt gactgttata tctatgaaat tcgatttgat ggtgtgaact ttcctgccaa
      421 tggtccagtt atgcagaaga ggactgtgaa atgggagcca tccactgaga aattgtatgt
      481 gcgtgatgga gtgctgaagg gtgatattaa cgcggctctg tcgcttgaag gaggtggcca
      541 ttaccgatgt gacttcaaaa ctacttataa agctaagaag gttgtccagt tgccagacta
      601 tcactttgtg gaccaccaca ttgagattaa aagccacgac aaagattaca gtaatgttaa
      661 tctgcatgag cacgccgaag cgcattctga gctgccgagg caggccaagt ccggactcag
      721 atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg
      781 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
      841 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
      901 ttgtttattg cagcttataa tgg
//
