LOCUS       sequence-85060-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             18..68
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             90..824
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gatccgctag cgccaccatg ggcgtggccg acttgatcaa gaagttcgag tccatctcca
       61 aggaggaggg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgatcaagg
      121 agaacatgca catgaagctg tacatggaag gcaccgtgaa caaccaccac ttcaagtgca
      181 ccaccgaagg ggagggcaag ccctacgagg gcacccagac ccagaggatt aaggtggtgg
      241 agggaggccc cctgccgttc gcattcgaca tcctggccac ctgctttatg tacgggagca
      301 agaccttcat caaccacacc cagggcatcc ccgatttctt taagcagtcc ttccctgagg
      361 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgcttacc gttacccagg
      421 acaccagcct ccaggacggc tgcttgatct acaacgtcaa gctcagaggg gtgaacttcc
      481 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccacc accgagaccc
      541 tgtaccccgc tgacggcggc ctggaaggca gatgcgacat ggccctgaag ctcgtgggcg
      601 ggggccacct gcactgcaac ctgaagacca catacagatc caagaaaccc gctaagaacc
      661 tcaagatgcc cggcgtctac tttgtggacc gcagactgga aagaatcaag gaggccgaca
      721 atgagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca
      781 aactggggca caaacttaat ggcatggacg agctgtacaa gtaagcggcc gcgactctag
      841 atcataatca gccataccac
//
