LOCUS       sequence-85061-e        1334 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-Peroxisomes-2
FEATURES             Location/Qualifiers
     source          1..1334
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        99..198
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     149..169
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    221..301
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      303..312
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             309..1016
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 gatgcggttt ggcagtacat caatgggcgt ggatagcggt tgactcacgg ggatttccaa
       61 gtctccaccc cattgacgtc aatgggagtt gtttggcacc aaaatcaacg ggactttcca
      121 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      181 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggactcag
      241 atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg
      301 tcgccaccat ggtgagcaag ggcgaggaga ccacaatggg cgtaatcaag cccgacatga
      361 agatcaagct gaagatggag ggcaacgtga atggccacgc cttcgtgatc gagggcgagg
      421 gcgagggcaa gccctacgac ggcaccaaca ccatcaacct ggaggtgaag gagggagccc
      481 ccctgccctt ctcctacgac attctgacca ccgcgttcgc ctacggcaac agggccttca
      541 ccaagtaccc cgacgacatc cccaactact tcaagcagtc cttccccgag ggctactctt
      601 gggagcgcac catgaccttc gaggacaagg gcatcgtgaa ggtgaagtcc gacatctcca
      661 tggaggagga ctccttcatc tacgagatac acctcaaggg cgagaacttc ccccccaacg
      721 gccccgtgat gcagaagaag accaccggct gggacgcctc caccgagagg atgtacgtgc
      781 gcgacggcgt gctgaagggc gacgtcaagc acaagctgct gctggagggc ggcggccacc
      841 accgcgttga cttcaagacc atctacaggg ccaagaaggc ggtgaagctg cccgactatc
      901 actttgtgga ccaccgcatc gagatcctga accacgacaa ggactacaac aaggtgaccg
      961 tttacgagag cgccgtggcc cgcaactcca ccgacggcat ggacgagctg tacaagtccg
     1021 gatccaagct gtagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg
     1081 ttttacttgc tttaaaaacc tcccacacct ccccctgaac ctgaaacata aatgaatgca
     1141 attgttgttg taacttgtta ttgcagctta taatggttac aataagcata gcatcacaaa
     1201 tttcacaata agcatttttt cactgcattc tagttgtgtt gtccaactca tcatgtatct
     1261 aagcgtaaat tgtagcgtat atttgtaaat cgcgtaattt tgtaatcagc tcattttaca
     1321 tagcgaatcg gcaa
//
