LOCUS       sequence-85064-e        1067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 584, Emission:
            607.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias 
            DA, Tsien RY
  TITLE     A monomeric red fluorescent protein.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7877-82.
  PUBMED    12060735
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2002-06-11"; volume: "99(12)"; pages: 
            "7877-82"
COMMENT     Sequence Label: mRFP1-pBAD
FEATURES             Location/Qualifiers
     source          1..1067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     86..105
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             188..210
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             230..247
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             251..283
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             296..316
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             323..1000
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             323..1000
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(453..471)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     721..740
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     915..938
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1046..1063)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 gccatgacaa aaacgggtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat
       61 tatttgcacg gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat
      121 actacctgac gcttttatcg caactctcta ctgtttctcc atacccgttt tttgggctag
      181 aaataatttt gtttaacttt aagaaggaga tatacatatg cggggttctc atcatcatca
      241 tcatcatggt atggctagca tgactggtgg acagcaaatg ggtcgggatc tgtacgagaa
      301 cctgtacttc cagggctcga gcatggcctc ctccgaggac gtcatcaagg agttcatgcg
      361 cttcaaggtg cgcatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
      421 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc
      481 cctgcccttc gcctgggaca tcctgtcccc tcagttccag tacggctcca aggcctacgt
      541 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
      601 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
      661 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
      721 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgga tgtaccccga
      781 ggacggcgcc ctgaagggcg agatcaagat gaggctgaag ctgaaggacg gcggccacta
      841 cgacgccgag gtcaagacca cctacatggc caagaagccc gtgcagctgc ccggcgccta
      901 caagaccgac atcaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca
      961 gtacgagcgc gccgagggcc gccactccac cggcgcctaa gaattcgaag cttggctgtt
     1021 ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaa
//
