LOCUS       sequence-85065-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 588, Emission: 635.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, 
            Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, 
            Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM
  TITLE     Far-red fluorescent tags for protein imaging in living tissues.
  JOURNAL   Biochem J. 2009 Mar 15;418(3):567-74. doi: 10.1042/BJ20081949.
  PUBMED    19143658
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2009-03-15- 15"; volume: "418"; issue: "3"; 
            pages: "567-74"
COMMENT     Sequence Label: mKate1.31-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..59
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     11..31
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             94..144
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      160..169
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             166..864
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
ORIGIN
        1 gccccattga cgcaaatggg cggtaggcgt gtacggtgga ggtctatata agcagagctg
       61 gtttagtgaa ccgtcagatc cgctagcgcc accatgggcg tggccgactt gatcaagaag
      121 ttcgagtcca tctccaagga ggagggggat ccaccggtcg ccaccatggt gagcgagctg
      181 attaaggaga acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc
      241 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
      301 gcggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac
      361 ggcagcaaaa ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc
      421 cccgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct
      481 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
      541 aacttcccat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggcctccacc
      601 gagaccctgt accccgctga cggcggcctg gaaggcagag ccgacatggc cctgaagctc
      661 gtgggcgggg gccacctgat ctgcaacttg aagaccacat acagatccaa gaaacccgct
      721 aagaacctca agatgcccgg cgtctactat gtggacagaa gactggaaag aatcaaggag
      781 gccgacaaag agacctacgt cgagcagcac gaggtggctg tggccagata ctgcgacctc
      841 cctagcaaac tggggcacag atgagcggcc gcgactctag atcataatca gccataccac
      901 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctcccccctg aacctgaaac
      961 ataaaatgaa tgcaattgtt gttgttaact tgtt
//
