LOCUS       sequence-85066-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKOet-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..60
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     11..31
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    83..163
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      165..174
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             198..848
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
       61 ggtttagtga accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc
      121 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca
      181 agggcgagga gaataacatg gccgtgatta aaccagagat gaagatgagg tactacatgg
      241 acggctccgt caatgggcat gagttcacaa ttgaaggtga aggcacaggc agaccttacg
      301 agggacatca agagatgaca ctacgcgtca caatggccaa gggcgggcca atgcctttcg
      361 cgtttgactt agtgtcacac gtgttctgtt acggccacag accttttact aaatatccag
      421 aagagatacc agactatttc aaacaagcat ttcctgaagg cctgtcatgg gaaaggtcgt
      481 tggagttcga agatggtggg tccgcttcag tcagtgcgca tataagcctt agaggaaaca
      541 ccttctacca caaatccaaa tttactgggg ttaactttcc tgccgatggt cctatcatgc
      601 aaaaccaaag tgttgattgg gagccatcaa ccgagaaaat tactgccagc gacggagttc
      661 tgaagggtga tgttacgatg tacctaaaac ttgaaggagg cggcaatcac aaatgccaat
      721 tcaagactac ttacaaggcg gcaaaaaaga ttcttaaaat gccaggaagc cattacatca
      781 gccatcgcct cgtcaggaaa accgaaggca acattactga gctggtagaa gatgcagtag
      841 ctcattccgg cggcatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc
      901 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
      961 aacctgaaac ataaaatgaa tgcaattgtt gttg
//
