LOCUS       sequence-85067-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKOet-C1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..60
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     11..31
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             132..782
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
     misc_feature    807..872
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
       61 ggtttagtga accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg
      121 aggagaataa catggccgtg attaaaccag agatgaagat gaggtactac atggacggct
      181 ccgtcaatgg gcatgagttc acaattgaag gtgaaggcac aggcagacct tacgagggac
      241 atcaagagat gacactacgc gtcacaatgg ccaagggcgg gccaatgcct ttcgcgtttg
      301 acttagtgtc acacgtgttc tgttacggcc acagaccttt tactaaatat ccagaagaga
      361 taccagacta tttcaaacaa gcatttcctg aaggcctgtc atgggaaagg tcgttggagt
      421 tcgaagatgg tgggtccgct tcagtcagtg cgcatataag ccttagagga aacaccttct
      481 accacaaatc caaatttact ggggttaact ttcctgccga tggtcctatc atgcaaaacc
      541 aaagtgttga ttgggagcca tcaaccgaga aaattactgc cagcgacgga gttctgaagg
      601 gtgatgttac gatgtaccta aaacttgaag gaggcggcaa tcacaaatgc caattcaaga
      661 ctacttacaa ggcggcaaaa aagattctta aaatgccagg aagccattac atcagccatc
      721 gcctcgtcag gaaaaccgaa ggcaacatta ctgagctggt agaagatgca gtagctcatt
      781 ccggcggcat ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttcgaatt
      841 ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatca
      901 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
      961 acctgaaaca taaatgaatg caattgttgt tgtt
//
