LOCUS       sequence-85070-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 450, Emission: 495.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: iLOV-N1
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        100..199
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     150..170
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    222..302
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    767..888
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(804..823)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     858..877
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
       61 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      121 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      181 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca
      241 gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg
      301 gtcgccacca tgatcgagaa gaacttcgtg atcaccgacc ccagactgcc cgacaacccc
      361 atcatcttcg ccagcgacgg cttcctggag ctgaccgagt acagcagaga ggagatcctg
      421 ggcagaaacg ccagattcct gcagggcccc gagaccgacc aggccaccgt gcagaagatc
      481 agagacgcca tcagagacca gagagagacc accgtgcagc tgatcaacta caccaagagc
      541 ggcaagaagt tctggaacct gctgcacctg cagcccgtga gagaccagaa gggcgagctg
      601 cagtacttca tcggcgtgca gctggacggc agcgaccacg tgtaagcggc cgcgactcta
      661 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
      721 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
      781 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      841 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg
      901 taagcgttaa tattttgtta aaa
//
