LOCUS       sequence-85074-e        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 580, Emission:
            608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-pBAD
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             106..128
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             148..165
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             169..201
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             214..234
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             241..939
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     primer_bind     complement(985..1002)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 gctatgccat agcattttat ccatagatta gcggatacta cctgacgctt ttatcgcaac
       61 tctctactgt tttctccata cccgtttttt tgggctagaa ataattttgt ttaactttaa
      121 gaaggagata tacatatgcg gggttctcat catcatcatc atcatggtat ggctagcatg
      181 actggtggac agcaaatggg tcgggatctg tacgagaacc tgtacttcca gggctcgagc
      241 atggtgagcg agctgattaa ggagaacatg cccatgaagc tgtacatgga gggcaccgtg
      301 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
      361 accatgagaa tcaaggtcgt cgagggcggc cctctcccct tcgccttcga catcctggct
      421 accagcttca tgtacggcag cagaaccttc atcaagcacc ctccgggcat ccccgacttc
      481 tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
      541 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
      601 aaggttagag gggtgaactt cccagccaac ggccctgtga tgcagaagaa aacactcggc
      661 tgggaggcct ccaccgagac gatgtacccc gctgacggcg gcctggaagg cgcatgtgac
      721 atggccctga agctcgtggg cgggggccac ctgatctgca accttgagac cacatacaga
      781 tccaagaaac ccgctacgaa cctcaagatg cccggcgtct acaacgtgga ccacagactg
      841 gaaagaatca aggaggccga cgatgagacc tacgtcgagc agcacgaggt ggctgtggcc
      901 agatactcta ctggtggcgc tggtgatgga ggtaaatgag aattcgaagc ttggctgttt
      961 tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct
     1021 gat
//
