LOCUS       sequence-85076-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mPlum-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        38..89
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     40..60
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      118..127
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             124..174
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             196..876
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     597..616
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     791..814
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ggactttcca aaatgtcgta acaactccgc ccccattgac gcaaatgggc ggtaggcgtg
       61 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgcc
      121 accatgggcg tggccgactt gatcaagaag ttcgagtcca tctccaagga ggagggggat
      181 ccaccggtgg ctaccatggt gagcaagggc gaggaggtca tcaaggagtt catgcgcttc
      241 aaggagcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      301 ggccgcccct acgagggcac ccagaccgcc aggctgaagg tgaccaaggg tggccccctg
      361 cccttcgcct gggacatcct gtcccctcag atcatgtacg gctccaaggc ctacgtgaag
      421 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
      481 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
      541 gacggcgagt tcatctacaa ggtgaaggtg cgcggcacca acttcccctc cgacggcccc
      601 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
      661 ggcgccctga agggcgagat gaagatgagg ctgaggctga aggacggcgg ccactacgac
      721 gccgaggtca agaccaccta catggccaag aagcccgtgc agctgcccgg cgcctacaag
      781 accgacatca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
      841 gagcgcgccg agggccgcca ctccaccggc gcctaagcgg ccgcgactct agatcataat
      901 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
      961 gaacctgaaa cataaaatga atgcaattgt tgtt
//
