LOCUS       sequence-85078-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: wtEos-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        38..89
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     40..60
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    112..192
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      194..203
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             200..883
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MVSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIA
                     RNDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDITMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
       61 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct
      121 accggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg
      181 ggatccaccg gtcgccacca tggtgagtgc gattaagcca gacatgaaga tcaacctccg
      241 tatggaaggc aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc
      301 ttttgaggga aaacagagta tggatcttga agtcaaagag ggcggacctc tgccttttgc
      361 ctttgatatc ctgaccactg cattccatta cggcaacagg gtattcgccg aatatccaga
      421 ccacatacaa gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt
      481 gactttcgaa gacgggggca tttgcattgc cagaaacgac ataacaatgg aaggggacac
      541 tttctataat aaagttcgat ttcacggtgt aaactttccc gccaatggtc cagttatgca
      601 gaagaagacg ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct
      661 gacgggtgat attaccatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt
      721 cagaactact tacaaagcta aggagaaggg tgtcaagtta ccaggctacc actttgtgga
      781 ccactgcatt gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca
      841 tgctgttgct cattctggat tgcctgacaa tgccagacga taagcggccg cgactctaga
      901 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
      961 tccccctgaa cctgaaacat aaaatgaatg caat
//
