LOCUS       sequence-85079-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Dronpa3-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        38..89
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     40..60
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    112..192
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      194..203
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             200..877
                     /codon_start=1
                     /product="photoswitchable Dronpa-Green3 fluorescent 
                     protein"
                     /label=Dronpa-Green3
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDINAALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
       61 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct
      121 accggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg
      181 ggatccaccg gtcgccacca tggtgagtgt gattaaacca gacatgaaga tcaagctgcg
      241 tatggaaggc gctgtaaatg gacacccgtt cgcgattgaa ggagttggcc ttgggaagcc
      301 tttcgaggga aaacagagta tggaccttaa agtcaaagaa ggcggacctc tgcctttcgc
      361 ctatgacatc ttgacaactg tgttctgtta cggcaacagg gtattcgcca aatacccaga
      421 aaatatagta gactatttca agcagtcgtt tcctgagggc tactcttggg aacgaagcat
      481 gaattacgaa gacgggggca tttgtaacgc gacaaacgac ataaccctgg atggtgactg
      541 ttatatctat gaaattcgat ttgatggtgt gaactttcct gccaatggtc cagttatgca
      601 gaagaggact gtgaaatggg agccatccac tgagaaattg tatgtgcgtg atggagtgct
      661 gaagggtgat attaacgcgg ctctgtcgct tgaaggaggt ggccattacc gatgtgactt
      721 caaaactact tataaagcta agaaggttgt ccagttgcca gactatcact ttgtggacca
      781 ccacattgag attaaaagcc acgacaaaga ttacagtaat gttaatctgc atgagcacgc
      841 cgaagcgcat tctgagctgc cgaggcaggc caagtaatga taggcggccg cgactctaga
      901 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaacc tcccacacct
      961 ccccctgaac ctgaaacata aatgaatgca attg
//
