LOCUS       sequence-85082-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mTagRFP-T2-N1
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        38..89
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     40..60
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    112..192
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      194..203
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             200..931
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTETLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
       61 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct
      121 accggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg
      181 ggatccaccg gtcgccacca tggtgtctaa gggcgaagag ctgattaagg agaacatgca
      241 catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca catccgaggg
      301 cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc
      361 tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca gaaccttcat
      421 caaccacacc cagggcatcc ccgatttctt taagcagtcc ttccctgagg gcttcacatg
      481 ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct
      541 ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc catccaacgg
      601 ccctgtgatg cagaagaaaa cactcggctg ggaggccaac accgagaccc tgtaccccgc
      661 tgacggcggc ctggaaggca gaaccgacat ggccctgaag ctcgtgggcg ggggccacct
      721 gatctgcaac ttcaagacca catacagatc caagaaaccc gctaagaacc tcaagatgcc
      781 cggcgtctac tatgtggacc acagactgga aagaatcaag gaggccgaca aagagaccta
      841 cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca aactggggca
      901 caaacttaat ggcatggacg agctgtacaa gtaagcggcc gcgactctag atcataatca
      961 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     1021 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gttta
//
