LOCUS       sequence-85084-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 400 / 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mPA-Emerald-C1
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        38..89
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     40..60
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      128..137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(134..136,137..139,140..850)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(179..200)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(440..459)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     787..808
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    851..916
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
       61 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct
      121 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      181 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      241 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      301 gccctggccc accctcgtga ccaccttgag ctacggcgtg cagtgcttcg cccgctaccc
      361 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      421 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      481 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      541 catcctgggg cacaagctgg agtacaacta caacagccac aaggtctata tcaccgccga
      601 caagcagaag aacggcatca aggtgaactt caagacccgc cacaacatcg aggacggcag
      661 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      721 gcccgacaac cactacctga gccaccagtc caagctgagc aaagacccca acgagaagcg
      781 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
      841 gctgtacaag tccggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac
      901 cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata ccacatttgt
      961 agaggtttta cttgctttaa aaacctccca cacctccccc tgaacctgaa cataaatgaa
     1021 tgcaattgtg tgtaacttgt tatgcagcta tatgctacaa taagc
//
