LOCUS       sequence-85086-e        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 490 / 553, Emission: 
            507 / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Dendra1-C1
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        52..151
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     102..122
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      190..199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    886..951
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(1112..1131)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
       61 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      121 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
      181 ctaccggtcg ccaccatgga cccgggtacc gtcaatctga ttaaagaaga tatgagggtt
      241 aaggtgcata tggaagggaa tgtaaacggg catgcttttg tgattgaagg ggaaggaaaa
      301 ggaaagccct acgaagggac acagaccgcg aacctgacag tgaaagaagg cgcgcctctc
      361 ccattttctt acgacatctt gacaacagca gtgcactacg gaaacagagt attcaccaaa
      421 taccctgaag atataccgga ttatttcaag cagtcgtttc ctgaaggata ttcctgggaa
      481 agaaccatga cttttgaaga caagggcatt tgtaccatca gaagcgacat aagcttggaa
      541 ggtgactgct ttttccaaaa cgttcgtttt aaggggacca actttccccc aaatggtcca
      601 gttatgcaga agaaaacttt gaagtgggaa ccatccacag agaagctgca cgtgcgtgat
      661 gggttgcttg tcggtaatat taacatggct ctgctgcttg aaggaggtgg acattacctg
      721 tgtgacttca aaactactta caaagcgaag aaggttgttc agttgccaga tgcccatttt
      781 gtggaccatc gcattgagat cttgggtaat gacagcgatt acaacaaagt gaagctgtac
      841 gagcatgccg ttgctcgcta ttctccgttg ccaagtcagg cctggtccgg actcagatct
      901 cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct
      961 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     1021 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     1081 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaa
//
