LOCUS       sequence-85087-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Dronpa-Peroxisomes-2
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..43
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    66..146
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      148..157
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             154..828
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
       61 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattct gcagtcgacg
      121 gtaccgcggg cccgggatcc accggtcgcc accatggtga gtgtgattaa accagacatg
      181 aagatcaagc tgcgtatgga aggcgctgta aatggacacc cgttcgcgat tgaaggagtt
      241 ggccttggga agcctttcga gggaaaacag agtatggacc ttaaagtcaa agaaggcgga
      301 cctctgcctt tcgcctatga catcttgaca actgtgttct gttacggcaa cagggtattc
      361 gccaaatacc cagaaaatat agtagactat ttcaagcagt cgtttcctga gggctactct
      421 tgggaacgaa gcatgaatta cgaagacggg ggcatttgta acgcgacaaa cgacataacc
      481 ctggatggtg actgttatat ctatgaaatt cgatttgatg gtgtgaactt tcctgccaat
      541 ggtccagtta tgcagaagag gactgtgaaa tgggagccat ccactgagaa attgtatgtg
      601 cgtgatggag tgctgaaggg tgatgttaac atggctctgt cgcttgaagg aggtggccat
      661 taccgatgtg acttcaaaac tacttataaa gctaagaagg ttgtccagtt gccagactat
      721 cactttgtgg accaccacat tgagattaaa agccacgaca aagattacag taatgttaat
      781 ctgcatgagc acgccgaagc gcattctgag ctgccgaggc aggccaagtc cggatccaag
      841 ctgtagcggc cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt
      901 gctttaaaaa aacctcccac acctcccccc tgaacctgaa acataaaatg aatgcaattg
      961 ttgttgttaa cttgtttatt gcagcttata atgg
//
