LOCUS       sequence-85088-e         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mCerulean-Peroxisomes-2
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..39
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    62..142
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      144..153
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(150..152,153..155,156..866)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(195..216)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(456..475)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     803..824
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc
       61 cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac
      121 cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
      181 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      241 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      301 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt
      361 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      421 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      481 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      541 tcaaggagga cggcaacatc ctggggcaca agctggagta caacgccatc agcgacaacg
      601 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
      661 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      721 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag
      781 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      841 ctctcggcat ggacgagctg tacaagtccg gatccaagct gtagcggccg cgactctaga
      901 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
      961 tcccc
//
