LOCUS       sequence-85090-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya1-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             23..73
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..808
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSYLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMCLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 ggtcagatcc gctagcgcca ccatgggcgt ggccgacttg atcaagaagt tcgagtccat
       61 ctccaaggag gagggggatc caccggtcgc caccatggtg agcaagggcg aggggcaatc
      121 gaagcatgga ctaaaggagg agatgacggt gaagtaccac atggaaggtt gcgttaatgg
      181 tcacaaattt gtcattactg gagagggcat tggaaaccct tttaagggta agcaaaccgc
      241 aaatttgtgt gtgatagaag gaggcccgct gccgttctcg gaggacattt taagtccggg
      301 ttttaaatat ggtgaccgga ttttcacaga gtacccacag gatattgtag attacttcaa
      361 gaactcatgt ccagcgggct atacgtggga aaggagctac ctctttgagg acggagcggt
      421 ctgtcgatgc aacgtggaca taacagtctc tgaaaaggag aactgcatct atcacaaaag
      481 tatcttcaga ggggtgaatt ttcccgccga cggccccgta atgaagaaaa tgaccactaa
      541 ttgggaagct agtaccgaga aaattgtgcc tgttccaaag caagggatat taaagggaaa
      601 ggtcaaaatg tgcctgttgc tgaaggatgg cggtcgttat cattgccagt ttgatacggt
      661 atataaagct aaatcagtgc cctccaagat gccagaatgg cattttatac agcataagct
      721 ccttagggag gaccgctctg atgctaaaaa ccagaaatgg caattaacag aacatgcaat
      781 cgcaggcatg gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata
      841 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
      901 aacataaaat gaatgcaatt gtt
//
