LOCUS       sequence-85091-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Dendra2-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..85
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             107..799
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 ggtttagtga accgtcagat ccgctagcgc caccatgggc gtggccgact tgatcaagaa
       61 gttcgagtcc atctccaagg aggaggggga tccaccggtc gccaccatga acaccccggg
      121 aattaacctg atcaaggagg acatgcgcgt gaaggtgcac atggagggca acgtgaacgg
      181 ccacgccttc gtgatcgagg gcgagggcaa gggcaagccc tacgagggca cccagaccgc
      241 caacctgacc gtgaaggagg gcgcccccct gcccttcagc tacgacatcc tgaccaccgc
      301 cgtgcactac ggcaaccggg tgttcaccaa gtaccccgag gacatccccg actacttcaa
      361 gcagagcttc cccgagggct acagctggga gcgcaccatg accttcgagg acaagggcat
      421 ctgcaccatc cgcagcgaca tcagcctgga gggcgactgc ttcttccaga acgtgcgctt
      481 caagggcacc aacttccccc ccaacggccc cgtgatgcag aagaagaccc tgaagtggga
      541 gcccagcacc gagaagctgc acgtgcgcga cggcctgctg gtgggcaaca tcaacatggc
      601 cctgctgctg gagggcggcg gccactacct gtgcgacttc aagaccacct acaaggccaa
      661 gaaggtggtg cagctgcccg acgcccactt cgtggaccac cgcatcgaga tcctgggcaa
      721 cgacagcgac tacaacaagg tgaagctgta cgagcacgcc gtggcccgct acagccccct
      781 gcccagccag gtgtggtaag cggccgcgac tctagatcat aatcagccat accacatttg
      841 tagaggtttt ac
//
