LOCUS       sequence-85092-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 400 / 490, Emission: 468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PS-CFP2-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        20..71
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     22..42
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..156
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             178..894
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
       61 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ccaccatggg cgtggccgac
      121 ttgatcaaga agttcgagtc catctccaag gaggaggggg atccaccggt cgccaccatg
      181 agcaagggcg ccgagctgtt caccggcatc gtgcccatcc tgatcgagct gaatggcgat
      241 gtgaatggcc acaagttcag cgtgagcggc gagggcgagg gcgatgccac ctacggcaag
      301 ctgaccctga agttcatctg caccaccggc aagctgcctg tgccctggcc caccctggtg
      361 gccaccctga gctacggcgt gcagtgcttc tcacgctacc ccgatcacat gaagcagcac
      421 gacttcttca agagcgccat gcctgagggc tacatccagg agcgcaccat cttcttcgag
      481 gatgacggca actacaagac gcgcgccgag gtgaagttcg agggcgatac cctggtgagt
      541 cgcatcgagc tgaccggcac tgatttcaag gaggatggca acatcctggg caataagatg
      601 gagtacaact acaacgccac caatgtgtac atcgtggccg acaaggccag gaatggcatc
      661 aaggtgaact tcaaggtccg ccacaacatc aaggatggca gcgtgcagct ggccgaccac
      721 taccagcaga atacccccat cggcgatggc cctgtgctgc tgcccgataa ccactacctg
      781 tccacccaga gcgccctgtc caaggacccc aacgagaagc gcgatcacat gatctacctc
      841 gagttcgtga ccgccgccgc catcacccac ggcatggatg agctgtacaa gtaaagcggc
      901 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
      961 acctcccaca cctccccctg aacctgaaac ataa
//
