LOCUS       sequence-85094-e         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 588, Emission: 635.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKate-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        20..71
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     22..42
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..156
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      172..181
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             178..891
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
       61 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ccaccatggg cgtggccgac
      121 ttgatcaaga agttcgagtc catctccaag gaggaggggg atccaccggt cgccaccatg
      181 gtgtctaagg gcgaagagct gattaaggag aacatgcaca tgaagctgta catggagggc
      241 accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
      301 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
      361 ctggctacca gcttcatgta cggcagcaaa accttcatca accacaccca gggcatcccc
      421 gacttcttta agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
      481 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
      541 aacgtcaaga tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca
      601 ctcggctggg aggcctccac cgagatgctg taccccgctg acggcggcct ggaaggcaga
      661 agcgacatgg ccctgaagct cgtgggcggg ggccacctga tctgcaactt gaagaccaca
      721 tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctacta tgtggacaga
      781 agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgaggtggct
      841 gtggccagat actgcgacct ccctagcaaa ctggggcaca aacttaattg agcggccgcg
      901 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
      961 cccacacctc cccctgaacc tgaacata
//
