LOCUS       sequence-85098-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 529, Emission: 539.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ZsYellow1-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        20..71
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     22..42
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    94..174
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      176..185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             182..877
                     /codon_start=1
                     /product="Zoanthus yellow fluorescent protein"
                     /label=ZsYellow1
                     /note="mammalian codon-optimized"
                     /translation="MAHSKHGLKEEMTMKYHMEGCVNGHKFVITGEGIGYPFKGKQTIN
                     LCVIEGGPLPFSEDILSAGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWGRSFLFEDGAV
                     CICNVDITVSVKENCIYHKSIFNGVNFPADGPVMKKMTTNWEASCEKIMPVPKQGILKG
                     DVSMYLLLKDGGRYRCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKWQLTEH
                     AIAFPSALA"
ORIGIN
        1 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
       61 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact cagatctcga
      121 gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac cggtcgccac
      181 catggcccac agcaagcacg gcctgaagga ggagatgacc atgaagtacc acatggaggg
      241 ctgcgtgaac ggccacaagt tcgtgatcac cggcgagggc atcggctacc ccttcaaggg
      301 caagcagacc atcaacctgt gcgtgatcga gggcggcccc ctgcccttca gcgaggacat
      361 cctgagcgcc ggcttcaagt acggcgaccg gatcttcacc gagtaccccc aggacatcgt
      421 ggactacttc aagaacagct gccccgccgg ctacacctgg ggccggagct tcctgttcga
      481 ggacggcgcc gtgtgcatct gtaacgtgga catcaccgtg agcgtgaagg agaactgcat
      541 ctaccacaag agcatcttca acggcgtgaa cttccccgcc gacggccccg tgatgaagaa
      601 gatgaccacc aactgggagg ccagctgcga gaagatcatg cccgtgccta agcagggcat
      661 cctgaagggc gacgtgagca tgtacctgct gctgaaggac ggcggccggt accggtgcca
      721 gttcgacacc gtgtacaagg ccaagagcgt gcccagcaag atgcccgagt ggcacttcat
      781 ccagcacaag ctgctgcggg aggaccggag cgacgccaag aaccagaagt ggcagctgac
      841 cgagcacgcc atcgccttcc ccagcgccct ggcctgaagc ggccgcgact ctagatcata
      901 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
      961 ctgaacctga aacataaaat gaatgcaatt gttg
//
