LOCUS       sequence-85099-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 493, Emission: 505.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ZsGreen1-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        20..71
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     22..42
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    94..174
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      176..185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             182..877
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
ORIGIN
        1 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
       61 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact cagatctcga
      121 gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac cggtcgccac
      181 catggcccag tccaagcacg gcctgaccaa ggagatgacc atgaagtacc gcatggaggg
      241 ctgcgtggac ggccacaagt tcgtgatcac cggcgagggc atcggctacc ccttcaaggg
      301 caagcaggcc atcaacctgt gcgtggtgga gggcggcccc ttgcccttcg ccgaggacat
      361 cttgtccgcc gccttcatgt acggcaaccg cgtgttcacc gagtaccccc aggacatcgt
      421 cgactacttc aagaactcct gccccgccgg ctacacctgg gaccgctcct tcctgttcga
      481 ggacggcgcc gtgtgcatct gcaacgccga catcaccgtg agcgtggagg agaactgcat
      541 gtaccacgag tccaagttct acggcgtgaa cttccccgcc gacggccccg tgatgaagaa
      601 gatgaccgac aactgggagc cctcctgcga gaagatcatc cccgtgccca agcagggcat
      661 cttgaagggc gacgtgagca tgtacctgct gctgaaggac ggtggccgct tgcgctgcca
      721 gttcgacacc gtgtacaagg ccaagtccgt gccccgcaag atgcccgact ggcacttcat
      781 ccagcacaag ctgacccgcg aggaccgcag cgacgccaag aaccagaagt ggcacctgac
      841 cgagcacgcc atcgcctccg gctccgcctt gccctgagcg gccgcgactc tagatcataa
      901 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
      961 tgaacctgaa acataaaatg aatgcaattg ttgt
//
