LOCUS       sequence-85100-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 576, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AsRed2-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        20..71
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     22..42
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    94..174
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      176..185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             182..880
                     /codon_start=1
                     /product="Anemonia sulcata red fluorescent protein"
                     /label=AsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASLLKKTMPFRTTIEGTVNGHYFKCTGKGEGNPLEGTQEMKIEV
                     IEGGPLPFAFHILSTSCMYGSKAFIKYVSGIPDYFKQSLPEGFTWERTTTYEDGGFLTA
                     HQDTSLDGDCLVYKVKILGNNFPADGPVMQNKAGRWEPSTEIVYEVDGVLRGQSLMALE
                     CPGGRHLTCHLHTTYRSKKPASALKMPGFHFEDHRIEILEEVEKGKCYKQYEAAVGRYC
                     DAAPSKLGHN"
ORIGIN
        1 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
       61 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact cagatctcga
      121 gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac cggtcgccac
      181 catggcctct ttgctgaaga agaccatgcc cttcaggacc accatcgagg gcaccgtgaa
      241 cggccactac ttcaagtgca ccggcaaggg cgagggcaac cccctggagg gcacccagga
      301 gatgaagatc gaggtgatcg agggcggccc cctgcccttc gccttccaca tcctgtccac
      361 ctcctgcatg tacggctcca aggccttcat caagtacgtg tccggcatcc ccgactactt
      421 caagcagtcc ctccccgagg gcttcacctg ggagcgcacc accacctacg aggacggcgg
      481 cttcctgacc gcccaccagg acacctccct ggacggcgac tgcctggtgt acaaggtgaa
      541 gatcctgggc aacaacttcc ccgccgacgg ccccgtgatg cagaacaagg ccggccgctg
      601 ggagccctcc accgagatcg tgtacgaggt ggacggcgtg ctgcgcggcc agtccctgat
      661 ggccctggag tgccccggcg gtcgccacct gacctgccac ctgcacacca cctaccgctc
      721 caagaagccc gcctccgccc tgaagatgcc cggcttccac ttcgaggacc accgcatcga
      781 gatcctggag gaggtggaga agggcaagtg ctacaagcag tacgaggccg ccgtgggccg
      841 ctactgcgac gccgccccct ccaagctggg ccacaactga agcggccgcg actctagatc
      901 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
      961 cccctgaacc tgaaacataa aatgaatgca attg
//
