LOCUS       sequence-85102-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 475, Emission: 505.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AcGFP1-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        20..71
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     22..42
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    94..174
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      176..185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             182..901
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
ORIGIN
        1 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
       61 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact cagatctcga
      121 gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac cggtcgccac
      181 catggtgagc aagggcgccg agctgttcac cggcatcgtg cccatcctga tcgagctgaa
      241 tggcgatgtg aatggccaca agttcagcgt gagcggcgag ggcgagggcg atgccaccta
      301 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcctgtgc cctggcccac
      361 cctggtgacc accctgagct acggcgtgca gtgcttctca cgctaccccg atcacatgaa
      421 gcagcacgac ttcttcaaga gcgccatgcc tgagggctac atccaggagc gcaccatctt
      481 cttcgaggat gacggcaact acaagtcgcg cgccgaggtg aagttcgagg gcgataccct
      541 ggtgaatcgc atcgagctga ccggcaccga tttcaaggag gatggcaaca tcctgggcaa
      601 taagatggag tacaactaca acgcccacaa tgtgtacatc atgaccgaca aggccaagaa
      661 tggcatcaag gtgaacttca agatccgcca caacatcgag gatggcagcg tgcagctggc
      721 cgaccactac cagcagaata cccccatcgg cgatggccct gtgctgctgc ccgataacca
      781 ctacctgtcc acccagagcg ccctgtccaa ggaccccaac gagaagcgcg atcacatgat
      841 ctacttcggc ttcgtgaccg ccgccgccat cacccacggc atggatgagc tgtacaagta
      901 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
      961 taaaaaacct cccacacctc cccctgaacc tgaa
//
