LOCUS       sequence-85109-e        1212 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 400 / 504, Emission: 
            515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mPA-GFP-N1
FEATURES             Location/Qualifiers
     source          1..1212
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        75..174
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     125..145
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    197..277
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      279..288
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(285..287,288..290,291..1001)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(330..351)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(591..610)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     938..959
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtggatagcg gtttgactca cggggatttt ccaagtctcc acccccattg acgtcaatgg
       61 gagtttgttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      121 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
      181 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
      241 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaagggcg
      301 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      361 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      421 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttca
      481 gctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
      541 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      601 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      661 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      721 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggccaact
      781 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
      841 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agccaccagt
      901 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
      961 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta
     1021 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa cctcccacac
     1081 ctcccctgac ctgaacataa atgatgcatt gtgtgtaact tgttattgca gctatatggt
     1141 tacaataagc atacatcaca attcacaata agcatttttc actgcatcta gtgtggttgc
     1201 caactcatca tg
//
