LOCUS       sequence-85111-e        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 600, Emission:
            650.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Lin MZ, McKeown MR, Ng HL, Aguilera TA, Shaner NC, Campbell RE, 
            Adams SR, Gross LA, Ma W, Alber T, Tsien RY
  TITLE     Autofluorescent proteins with excitation in the optical window for 
            intravital imaging in mammals.
  JOURNAL   Chem Biol. 2009 Nov 25;16(11):1169-79. doi: 
            10.1016/j.chembiol.2009.10.009.
  PUBMED    19942140
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2009.10"; journalName: "Chem Biol"; date: 
            "2009-11-25- 25"; volume: "16"; issue: "11"; pages: "1169-79"
COMMENT     Sequence Label: mNeptune-pBAD
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             177..194
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             198..230
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(234..242,243..257)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             273..1007
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein derived 
                     from mKate"
                     /label=mNeptune
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTG
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRCD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gttctatatc acgcagaaag tcacatgatt atttgcacgg cgtcacactt tgctatgcca
       61 tagcattttt atccataaga ttagcggatc ctacctgacg cttttatcgc aactctctac
      121 tgtttctcca tacccgtttt ttgggctaac aggaggaatt aaccatgggg ggttctcatc
      181 atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt cgggatctgt
      241 acgacgatga cgataaggat ccgagctcga gcatggtgag caagggcgaa gagctgatta
      301 aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt
      361 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccggcaga atcaaggtgg
      421 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tacctgcttc atgtacggca
      481 gcaagacctt catcaaccac acccagggca tccccgattt ctttaagcag tccttccctg
      541 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc
      601 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact
      661 tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc agcaccgaga
      721 ccctgtaccc cgctgacggc ggcctggaag gcagatgcga catggccctg aagctcgtgg
      781 gcgggggcca cctgatctgc aacctgaaga ccacatacag atccaagaaa cccgctaaga
      841 acctcaagat gcccggcgtc tactttgtgg accgcagact ggaaagaatc aaggaggccg
      901 acaaagagac ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta
      961 gcaaactggg gcacaaactt aatggcatgg acgagctgta caagtaagaa ttcgaagctt
     1021 ggctgttttg gcggatgaga gaagattttc a
//
