LOCUS       sequence-85113-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya-N1
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        13..112
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     63..83
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    135..215
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      217..226
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             223..936
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSFLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMYLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 gtttgtttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
       61 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
      121 gaaccgtcag atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattctg
      181 cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
      241 gggcaatcga agcatgggct aaaggaggag atgacggtga agtaccacat ggaaggttgc
      301 gttaatggtc acaaatttgt cattactgga gagggcattg gaaacccttt taagggtaag
      361 caaactgcaa atttgtgtgt gatagaagga ggcccgctgc cgttctcgga ggacatttta
      421 agtccgggtt ttaaatatgg tgaccggatt ttcacagagt acccacagga tattgtagat
      481 tacttcaaga actcatgtcc agcgggctat acgtgggaaa ggagcttcct ctttgaggac
      541 ggagcggtct gtcgatgcaa cgtggacata acagtctctg aaaaggagaa ctgcatctat
      601 cacaaaagta tcttcagagg ggtgaatttt cccgccgacg gccccgtaat gaagaaaatg
      661 accactaatt gggaagctag taccgagaaa attgtgcctg ttccaaagca agggatatta
      721 aaaggaaagg tcaaaatgta cctattgctg aaggatggcg gtcgttatca ttgccagttt
      781 gatacggtat ataaagctaa atcagtgccc tccaagatgc cagaatggca ttttatacag
      841 cataagctcc ttagggagga ccgctctgat gctaaaaacc agaaatggca attaacagaa
      901 catgcaatcg caggcatgga cgagctgtac aagtaagcgg ccgcgactct agatcataat
      961 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     1021 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtt
//
