LOCUS       sequence-85114-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 514, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mTopaz-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        19..70
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     21..41
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    93..173
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      175..184
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(181..183,184..186,187..900)
                     /codon_start=1
                     /product="enhanced yellow variant of GFP"
                     /label=Topaz YFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMRQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(226..247)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(487..506)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     834..855
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
       61 aagcagagct ggtttagtga accgtcagat ccgctagcgc taccggactc agatctcgag
      121 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc
      181 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      241 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      301 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      361 ctcgtgacca ccttcggcta cggcgtgcag tgcttcgccc gctaccccga ccacatgcgc
      421 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      481 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      541 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      601 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      661 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      721 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      781 tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      841 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
      901 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
      961 aaaaaaacct cccacacctc ccccctgaac ctga
//
