LOCUS       sequence-85115-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 507 / 583, Emission: 
            517 / 593.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: wtKikGR-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        19..70
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     21..41
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    93..173
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      175..184
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             181..867
                     /codon_start=1
                     /product="photoconvertible Kikume green-to-red fluorescent 
                     protein (Tsutsui et al., 2005)"
                     /label=KikGR1
                     /translation="MVSVITSEMKIELRMEGAVNGHKFVITGKGSGQPFEGIQNVDLTV
                     IEGGPLPFAFDILTTAFHYGNRVFVEYPEEIVDYFKQSFPEGYSWERSMSYEDGGICLA
                     TNNITMKKDGSNCFVNEIRFDGVNFPANGPVMQRKTVKWEPSTEKMYVRDGVLKGDVNM
                     ALLLQGGGHYRCDFRTTYKAKKVVQLPDYHFVDHQMEITSHDKDYNKVKLYEHAKAHSG
                     LPRLAK"
ORIGIN
        1 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
       61 aagcagagct ggtttagtga accgtcagat ccgctagcgc taccggactc agatctcgag
      121 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc
      181 atggtgagtg tgattacatc agaaatgaag atcgagctgc gtatggaagg cgctgtaaac
      241 gggcacaagt tcgtgattac agggaaagga agtggccagc ctttcgaggg aatacagaat
      301 gtggacctga cagtcataga gggcggacct cttccttttg ctttcgatat cctgacaaca
      361 gcattccatt acggcaaccg ggtatttgtc gaatacccag aagaaatagt agactacttc
      421 aagcagtcgt ttcctgaggg ttattcttgg gaacgaagca tgagttacga agacggggga
      481 atttgcctcg ccacaaacaa tataacgatg aagaaagacg gcagcaactg ttttgtcaat
      541 gaaattcgat ttgatggtgt gaactttcct gccaatggtc cagttatgca gaggaagacc
      601 gtcaaatggg agccatccac tgagaaaatg tatgtgcgtg atggagtgct gaagggtgat
      661 gtaaacatgg ctctgttgct tcaaggaggt ggccattacc gatgtgactt cagaactact
      721 tacaaagcaa agaaggttgt ccagttgcca gactatcact tcgtggatca tcaaatggag
      781 ataacaagcc atgacaagga ttacaacaag gttaagctgt atgagcacgc taaagctcat
      841 tccgggctgc caaggctggc caagtaagcg gccgcgactc tagatcataa tcagccatac
      901 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
      961 acataaaatg aatgcaattg ttgttgttaa cttg
//
