LOCUS       sequence-85119-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya1-C1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      68..77
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             74..784
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSYLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMCLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
     misc_feature    785..850
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct
       61 accggtcgcc accatggtga gcaagggcga ggggcaatcg aagcatggac taaaggagga
      121 gatgacggtg aagtaccaca tggaaggttg cgttaatggt cacaaatttg tcattactgg
      181 agagggcatt ggaaaccctt ttaagggtaa gcaaaccgca aatttgtgtg tgatagaagg
      241 aggcccgctg ccgttctcgg aggacatttt aagtccgggt tttaaatatg gtgaccggat
      301 tttcacagag tacccacagg atattgtaga ttacttcaag aactcatgtc cagcgggcta
      361 tacgtgggaa aggagctacc tctttgagga cggagcggtc tgtcgatgca acgtggacat
      421 aacagtctct gaaaaggaga actgcatcta tcacaaaagt atcttcagag gggtgaattt
      481 tcccgccgac ggccccgtaa tgaagaaaat gaccactaat tgggaagcta gtaccgagaa
      541 aattgtgcct gttccaaagc aagggatatt aaagggaaag gtcaaaatgt gcctgttgct
      601 gaaggatggc ggtcgttatc attgccagtt tgatacggta tataaagcta aatcagtgcc
      661 ctccaagatg ccagaatggc attttataca gcataagctc cttagggagg accgctctga
      721 tgctaaaaac cagaaatggc aattaacaga acatgcaatc gcaggcatgg acgagctgta
      781 caagtccgga ctcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg
      841 cccgggatcc accggatcta gataactgat cataatcagc cataccacat ttgtagaggt
      901 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
      961 aattgttgtt gttaacttgt ttattgcagc ttat
//
