LOCUS       sequence-85121-e        1200 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C2 Cloning Vector, Excitation: 490 / 553, Emission: 
            507 / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Dendra1-C2
FEATURES             Location/Qualifiers
     source          1..1200
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        69..168
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     119..139
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      207..216
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    916..972
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    1096..1177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1133..1152)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
       61 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      121 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
      181 cgtcagatcc gctagcgcta ccggtcgcca ccatggaccc gggtaccgtc aatctgatta
      241 aagaagatat gagggttaag gtgcatatgg aagggaatgt aaacgggcat gcttttgtga
      301 ttgaagggga aggaaaagga aagccctacg aagggacaca gaccgcgaac ctgacagtga
      361 aagaaggcgc gcctctccca ttttcttacg acatcttgac aacagcagtg cactacggaa
      421 acagagtatt caccaaatac cctgaagata taccggatta tttcaagcag tcgtttcctg
      481 aaggatattc ctgggaaaga accatgactt ttgaagacaa gggcatttgt accatcagaa
      541 gcgacataag cttggaaggt gactgctttt tccaaaacgt tcgttttaag gggaccaact
      601 ttcccccaaa tggtccagtt atgcagaaga aaactttgaa gtgggaacca tccacagaga
      661 agctgcacgt gcgtgatggg ttgcttgtcg gtaatattaa catggctctg ctgcttgaag
      721 gaggtggaca ttacctgtgt gacttcaaaa ctacttacaa agcgaagaag gttgttcagt
      781 tgccagatgc ccattttgtg gaccatcgca ttgagatctt gggtaatgac agcgattaca
      841 acaaagtgaa gctgtacgag catgccgttg ctcgctattc tccgttgcca agtcaggcct
      901 ggtccggccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg
      961 ggcccgggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
     1021 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     1081 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     1141 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
//
