LOCUS       sequence-85128-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKO2-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        43..94
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     45..65
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      123..132
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             129..179
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      195..204
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             201..857
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
       61 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      121 gcgccaccat gggcgtggcc gacttgatca agaagttcga gtccatctcc aaggaggagg
      181 gggatccacc ggtcgccacc atggtgagtg tgattaaacc agagatgaag atgaggtact
      241 acatggacgg ctccgtcaat gggcatgagt tcacaattga aggtgaaggc acaggcagac
      301 cttacgaggg acatcaagag atgacactac gcgtcacaat ggccgagggc gggccaatgc
      361 ctttcgcgtt tgacttagtg tcacacgtgt tctgttacgg ccacagagta tttactaaat
      421 atccagaaga gataccagac tatttcaaac aagcatttcc tgaaggcctg tcatgggaaa
      481 ggtcgttgga gttcgaagat ggtgggtccg cttcagtcag tgcgcatata agccttagag
      541 gaaacacctt ctaccacaaa tccaaattta ctggggttaa ctttcctgcc gatggtccta
      601 tcatgcaaaa ccaaagtgtt gattgggagc catcaaccga gaaaattact gccagcgacg
      661 gagttctgaa gggtgatgtt acgatgtacc taaaacttga aggaggcggc aatcacaaat
      721 gccaaatgaa gactacttac aaggcggcaa aagagattct tgaaatgcca ggagaccatt
      781 acatcggcca tcgcctcgtc aggaaaaccg aaggcaacat tactgagcag gtagaagatg
      841 cagtagctca tagctaagcg gccgcgactc tagatcataa tcagccatac cacatttgta
      901 gaggttttac ttgctttaaa aaacctccca cacctccccc ctgaacctga aacataaaat
      961 gaatgcaatt gttgttgtta acttgtttat tgca
//
