LOCUS       sequence-85132-e        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 548, Emission:
            561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A
  TITLE     Cyan-emitting and orange-emitting fluorescent proteins as a 
            donor/acceptor pair for fluorescence resonance energy transfer.
  JOURNAL   Biochem J. 2004 Jul 1. 381(Pt 1):307-12.
  PUBMED    15065984
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2004 Jul 1. 381(Pt 1):307-12."
COMMENT     Sequence Label: mKO-pBAD
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             154..176
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             196..213
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             217..249
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             262..282
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             289..945
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
     primer_bind     complement(991..1008)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc
       61 attttatcca taagattagc ggatactacc tgacgctttt tatcgcaact ctctactgtt
      121 tctccatacc cgtttttttg ggctagaaat aattttgttt aactttaaga aggagatata
      181 catatgcggg gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag
      241 caaatgggtc gggatctgta cgagaacctg tacttccagg gctcgagcat ggtgagtgtg
      301 attaaaccag agatgaagat gaggtactac atggacggct ccgtcaatgg gcatgagttc
      361 acaattgaag gtgaaggcac aggcagacct tacgagggac atcaagagat gacactacgc
      421 gtcacaatgg ccaagggcgg gccaatgcct ttcgcgtttg acttagtgtc acacgtgttc
      481 tgttacggcc acagaccttt tactaaatat ccagaagaga taccagacta tttcaaacaa
      541 gcatttcctg aaggcctgtc atgggaaagg tcgttggagt tcgaagatgg tgggtccgct
      601 tcagtcagtg cgcatataag ccttagagga aacaccttct accacaaatc caaatttact
      661 ggggttaact ttcctgccga tggtcctatc atgcaaaacc aaagtgttga ttgggagcca
      721 tcaaccgaga aaattactgc cagcgacgga gttctgaagg gtgatgttac gatgtaccta
      781 aaacttgaag gaggcggcaa tcacaaatgc caattcaaga ctacttacaa ggcggcaaaa
      841 aagattctta aaatgccagg aagccattac atcagccatc gcctcgtcag gaaaaccgaa
      901 ggcaacatta ctgagctggt agaagatgca gtagctcatt cctaagaatt cgaagcttgg
      961 ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag
     1021 cgg
//
