LOCUS       sequence-85133-e        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 400 / 485, Emission: 
            468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PA-sfGFP-N1
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..136
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     87..107
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    159..239
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      241..250
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(247..249,250..252,253..966)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(292..313)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     900..921
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tccaccccat tgacgtcaat gggagtttgt ttggcaccaa aatcaacggg actttccaaa
       61 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      121 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
      181 ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggtc
      241 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      301 ctggacggcg acgtaaacgg ccacaagttc agcgtgcgcg gcgagggcga gggcgatgcc
      361 accaacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      421 cccaccctcg tgaccaccct gagctacggc gtgcagtgct tcagccgcta ccccgaccac
      481 atgaagcgcc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      541 atcagcttca aggacgacgg cacctacaag acccgcgccg aggtgaagtt cgagggcgac
      601 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      661 gggcacaagc tggagtacaa cttcaacagc cacaacgtct atatcaccgc cgacaagcag
      721 aagaacggca tcaaggccaa cttcaagatc cgccacaacg tggaggacgg cagcgtgcag
      781 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      841 aaccactacc tgagccacca gtccgtgctg agcaaagacc ccaacgagaa gcgcgatcac
      901 atggtcctgc tggagttcgt gaccgccgcc gggatcactc acggcatgga cgagctgtac
      961 aagtaagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact
     1021 tgctttaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     1081 gttgttaact tgttattgca gcttataatg gttacaataa agcatagcat cacaaa
//
