LOCUS       sequence-85134-e         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 485, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Pedelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS
  TITLE     Engineering and characterization of a superfolder green fluorescent 
            protein.
  JOURNAL   Nat Biotechnol. 2006 Jan;24(1):79-88. doi: 10.1038/nbt1172
  PUBMED    16369541
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2006-01"; volume: "24"; issue: "1"; pages: 
            "79-88"
COMMENT     Sequence Label: sfGFP-N1
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..104
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     55..75
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    127..207
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      209..218
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(215..217,218..220,221..934)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(260..281)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     868..889
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
       61 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      121 agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac
      181 ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt
      241 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      301 cgtgcgcggc gagggcgagg gcgatgccac caacggcaag ctgaccctga agttcatctg
      361 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
      421 gcagtgcttc agccgctacc ccgaccacat gaagcgccac gacttcttca agtccgccat
      481 gcccgaaggc tacgtccagg agcgcaccat cagcttcaag gacgacggca cctacaagac
      541 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      601 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact tcaacagcca
      661 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
      721 ccacaacgtg gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      781 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgtgctgag
      841 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      901 gatcactcac ggcatggacg agctgtacaa gtaagcggcc gcgactctag atcataatca
      961 gccataccac atttgtagag gttttacttg ctttaaaaa
//
