LOCUS       sequence-85137-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Rego EH, Shao L, Macklin JJ, Winoto L, Johansson GA, Kamps-Hughes N,
            Davidson MW, Gustafsson MG
  TITLE     Nonlinear structured-illumination microscopy with a photoswitchable 
            protein reveals cellular structures at 50-nm resolution.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 17;109(3):E135-43. doi: 
            10.1073/pnas.1107547108. Epub 2011 Dec 12.
  PUBMED    22160683
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1107547108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-01-17- 17"; volume: "109"; issue: "3"; pages: "E135-43"
COMMENT     Sequence Label: Dronpa-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        12..63
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     14..34
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      92..101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..148
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      164..173
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             170..847
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
       61 gctggtttag tgaaccgtca gatccgctag cgccaccatg ggcgtggccg acttgatcaa
      121 gaagttcgag tccatctcca aggaggaggg ggatccaccg gtcgccacca tggtgagtgt
      181 gattaaacca gacatgaaga tcaagctgcg tatggaaggc gctgtaaatg gacacccgtt
      241 cgcgattgaa ggagttggcc ttgggaagcc tttcgaggga aaacagagta tggaccttaa
      301 agtcaaagaa ggcggacctc tgcctttcgc ctatgacatc ttgacaactg tgttctgtta
      361 cggcaacagg gtattcgcca aatacccaga aaatatagta gactatttca agcagtcgtt
      421 tcctgagggc tactcttggg aacgaagcat gaattacgaa gacgggggca tttgtaacgc
      481 gacaaacgac ataaccctgg atggtgactg ttatatctat gaaattcgat ttgatggtgt
      541 gaactttcct gccaatggtc cagttatgca gaagaggact gtgaaatggg agccatccac
      601 tgagaaattg tatgtgcgtg atggagtgct gaagggtgat gttaacatgg ctctgtcgct
      661 tgaaggaggt ggccattacc gatgtgactt caaaactact tataaagcta agaaggttgt
      721 ccagttgcca gactatcact ttgtggacca ccacattgag attaaaagcc acgacaaaga
      781 ttacagtaat gttaatctgc atgagcacgc cgaagcgcat tctgagctgc cgaggcaggc
      841 caagtaatga taggcggccg cgactctaga tcataatcag ccataccaca tttgtagagg
      901 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
      961 caattgttgt tgttaacttg tttattgcag ctta
//
