LOCUS       sequence-85138-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 503 / 569, Emission: 
            514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mGeos2-VEL-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        12..63
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     14..34
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    86..166
                     /label=MCS
                     /note="multiple cloning site"
     CDS             174..854
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
       61 gctggtttag tgaaccgtca gatccgctag cgctaccgga ctcagatctc gagctcaagc
      121 ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatgagtg
      181 cgattaagcc agacatgaag atcaaactcc gtatggaagg caacgtaaac gggcaccact
      241 ttgtgatcga cggagatggt acaggcaagc cttttgaggg aaaacagagt atggatcttg
      301 aagtcaaaga gggcggacct ctgccttttg cctttgatat cctgaccact gcattcctgt
      361 acggcaacag ggtattcgcc aaatatccag acaacataca agactatttt aagcagtcgt
      421 ttcctaaggg gtattcgtgg gaacgaagct tgactttcga agacgggggc atttgcaacg
      481 ccagaaacga cataacaatg gaaggggaca ctttctataa taaagttcga ttttatggta
      541 ccaactttcc cgccaatggt ccagttatgc agaagaagac gctgaaatgg gagccctcca
      601 ctgagaaaat gtatgtgcgt gatggagtgc tgacgggtga tgtagagatg gctttgttgc
      661 ttgaaggaaa tgcccattac cgatgtgact tcagaactac ttacaaagct aaggagaagg
      721 gtgtcaagtt accaggcgcc cactttgtgg accactgcat tgagatttta agccatgaca
      781 aagattacaa caaggttaag ctgtatgagc atgctgttgc tcattctgga ttgcctgaca
      841 atgccagacg ataagcggcc gcgactctag atcataatca gccataccac atttgtagag
      901 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
      961 gcaattgttg ttgttaactt gtttattgca gctt
//
