LOCUS       sequence-85143-e         619 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 450, Emission: 495.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: iLOV-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..619
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        13..64
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     15..35
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             99..149
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
ORIGIN
        1 tccgccccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
       61 agctggttta gtgaaccgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca
      121 agaagttcga gtccatctcc aaggaggagg gggatccacc ggtcgccacc atgatcgaga
      181 agaacttcgt gatcaccgac cccagactgc ccgacaaccc catcatcttc gccagcgacg
      241 gcttcctgga gctgaccgag tacagcagag aggagatcct gggcagaaac gccagattcc
      301 tgcagggccc cgagaccgac caggccaccg tgcagaagat cagagacgcc atcagagacc
      361 agagagagac caccgtgcag ctgatcaact acaccaagag cggcaagaag ttctggaacc
      421 tgctgcacct gcagcccgtg agagaccaga agggcgagct gcagtacttc atcggcgtgc
      481 agctggacgg cagcgaccac gtgtaagcgg ccgcgactct agatcataat cagccatacc
      541 acatttgtag aggttttact tgctttaaaa aaacctccca cacctccccc tgaacctgaa
      601 acataaaatg aatgcaatt
//
